Description: Rabbit polyclonal antibody to CDKL3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDKL3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDKL3 Alternative Names: NKIAMRE; Cyclin-dependent kinase-like 3; Serine/threonine-protein kinase NKIAMREEntrez Gene (Human): 51265Entrez Gene (Mouse) : 213084Entrez Gene (Rat) : 60396SwissProt (Human): Q8IVW4SwissProt (Mouse) : Q8BLF2SwissProt (Rat) : Q9JM01Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDKL3 expression in mouse heart (A), rat heart (B) whole cell lysates.
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Immunohistochemical analysis of CDKL3 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.