Description: Rabbit polyclonal antibody to ANKRD1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ANKRD1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ANKRD1 Alternative Names: C193; CARP; HA1A2; Ankyrin repeat domain-containing protein 1; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear proteinEntrez Gene (Human): 27063Entrez Gene (Mouse) : 107765Entrez Gene (Rat) : 27064SwissProt (Human): Q15327SwissProt (Mouse) : Q9CR42SwissProt (Rat) : Q8R560Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ANKRD1 expression in mouse muscle (A), rat muscle (B) whole cell lysates.
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Immunohistochemical analysis of ANKRD1 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The muscle ankyrin repeat proteins are hypoxia-sensitive: in vivo mRNA expression in the hypoxia-tolerant blind subterranean mole rat, Spalax ehrenbergi