Description: Rabbit polyclonal antibody to CLIC4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CLIC4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CLIC4 Alternative Names: Chloride intracellular channel protein 4; Intracellular chloride ion channel protein p64H1Entrez Gene (Human): 25932Entrez Gene (Mouse) : 29876Entrez Gene (Rat) : 83718SwissProt (Human): Q9Y696SwissProt (Mouse) : Q9QYB1SwissProt (Rat) : Q9Z0W7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CLIC4 expression in COS7 (A), LOVO (B), CT26 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of CLIC4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.