Description: Rabbit polyclonal antibody to p116 Rip Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p116 Rip. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MPRIP Alternative Names: KIAA0864; MRIP; RHOIP3; Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116RipEntrez Gene (Human): 23164Entrez Gene (Mouse) : 26936Entrez Gene (Rat) : 116504SwissProt (Human): Q6WCQ1SwissProt (Mouse) : P97434SwissProt (Rat) : Q9ERE6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p116 Rip expression in PC12 (A), MEF (B), A549 (C), A2780 (D) whole cell lysates.
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Immunohistochemical analysis of p116 Rip staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.