Description: Rabbit polyclonal antibody to Histamine H3 Receptor Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H3 Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: HRH3 Alternative Names: GPCR97; Histamine H3 receptor; H3R; HH3R; G-protein coupled receptor 97Entrez Gene (Human): 11255Entrez Gene (Mouse) : 99296Entrez Gene (Rat) : 85268SwissProt (Human): Q9Y5N1SwissProt (Mouse) : P58406SwissProt (Rat) : Q9QYN8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histamine H3 Receptor expression in HEK293T (A), Hela (B), H460 (C) whole cell lysates.
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  Immunohistochemical analysis of Histamine H3 Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Histamine H3 Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.