Description: Rabbit polyclonal antibody to EMP Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EMP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MAEA Alternative Names: EMP; Macrophage erythroblast attacher; Cell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; Human lung cancer oncogene 10 protein; HLC-10Entrez Gene (Human): 10296Entrez Gene (Mouse) : 59003Entrez Gene (Rat) : 298982SwissProt (Human): Q7L5Y9SwissProt (Mouse) : Q4VC33SwissProt (Rat) : Q5RKJ1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EMP expression in HepG2 (A), HeLa (B) whole cell lysates.
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  Immunohistochemical analysis of EMP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.