Description: Rabbit polyclonal antibody to ZEB2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ZEB2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ZEB2 Alternative Names: KIAA0569; SIP1; ZFHX1B; ZFX1B; Zinc finger E-box-binding homeobox 2; Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1bEntrez Gene (Human): 9839SwissProt (Human): O60315Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ZEB2 expression in mouse liver (A) whole cell lysates.
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Immunohistochemical analysis of ZEB2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.