Description: Rabbit polyclonal antibody to CDKL2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDKL2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDKL2 Alternative Names: Cyclin-dependent kinase-like 2; Protein kinase p56 KKIAMRE; Serine/threonine-protein kinase KKIAMREEntrez Gene (Human): 8999SwissProt (Human): Q92772Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDKL2 expression in H9C2 (A), SKOVCAR3 (B), MCF7 (C) whole cell lysates.
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Immunohistochemical analysis of CDKL2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.