Description: Rabbit polyclonal antibody to PLA2G4C Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLA2G4C. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PLA2G4C Alternative Names: Cytosolic phospholipase A2 gamma; cPLA2-gamma; Phospholipase A2 group IVCEntrez Gene (Human): 8605SwissProt (Human): Q9UP65Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLA2G4C expression in Jurkat (A), H446 (B), mouse lung (C), rat lung (D), rat spleen (E) whole cell lysates.
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Immunohistochemical analysis of PLA2G4C staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
HDAC inhibition induces increased choline uptake and elevated phosphocholine levels in MCF7 breast cancer cells