Description: Rabbit polyclonal antibody to Tyrosine Hydroxylase (Phospho-S71) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S71 of human Tyrosine Hydroxylase protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TH Alternative Names: TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; THEntrez Gene (Human): 7054Entrez Gene (Mouse) : 21823Entrez Gene (Rat) : 25085SwissProt (Human): P07101SwissProt (Mouse) : P24529SwissProt (Rat) : P04177Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Tyrosine Hydroxylase (Phospho-S71) expression in HepG2 UV-treated (A), mouse brain (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of Tyrosine Hydroxylase (Phospho-S71) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Direct ELISA antibody dose-response curve using Anti-Tyrosine Hydroxylase (Phospho-S71) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.