Description: Rabbit polyclonal antibody to STAT4 (Phospho-Y693) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y693 of human STAT4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: STAT4 Alternative Names: Signal transducer and activator of transcription 4Entrez Gene (Human): 6775Entrez Gene (Mouse) : 20849SwissProt (Human): Q14765SwissProt (Mouse) : P42228Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAT4 (Phospho-Y693) expression in PMVEC (A) whole cell lysates.
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Immunohistochemical analysis of STAT4 (Phospho-Y693) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.