Description: Rabbit polyclonal antibody to PLC gamma 1 (Phospho-Y783) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y783 of human PLC gamma 1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PLCG1 Alternative Names: PLC1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1Entrez Gene (Human): 5335Entrez Gene (Mouse) : 18803Entrez Gene (Rat) : 25738SwissProt (Human): P19174SwissProt (Mouse) : Q62077SwissProt (Rat) : P10686Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLC gamma 1 (Phospho-Y783) expression in Jurkat pervanadate-treated (A), NIH3T3 LPS-treated (B), PC12 H2O2-treated (C) whole cell lysates.
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Immunohistochemical analysis of PLC gamma 1 (Phospho-Y783) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.