Description: Rabbit polyclonal antibody to NF-kappaB p105 (Phospho-S932) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S932 of human NF-kappaB p105 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NFKB1 Alternative Names: Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1Entrez Gene (Human): 4790Entrez Gene (Mouse) : 18033SwissProt (Human): P19838SwissProt (Mouse) : P25799SwissProt (Rat) : Q63369Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NF-kappaB p105 (Phospho-S932) expression in HeLa LPS-treated (A), NIH3T3 LPS-treated (B), Raw264.7 LPS-treated (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of NF-kappaB p105 (Phospho-S932) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.