Description: Rabbit polyclonal antibody to MYF6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MYF6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MYF6 Alternative Names: BHLHC4; MRF4; Myogenic factor 6; Myf-6; Class C basic helix-loop-helix protein 4; bHLHc4; Muscle-specific regulatory factor 4Entrez Gene (Human): 4618Entrez Gene (Mouse) : 17878Entrez Gene (Rat) : 25714SwissProt (Human): P23409SwissProt (Mouse) : P15375SwissProt (Rat) : P19335Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MYF6 expression in HEK293T (A), mouse brain (B), mouse muscle (C), rat muscle (D) whole cell lysates.
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Immunohistochemical analysis of MYF6 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.