Description: Rabbit polyclonal antibody to HCFC1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HCFC1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: HCFC1 Alternative Names: HCF1; HFC1; Host cell factor 1; HCF; HCF-1; C1 factor; CFF; VCAF; VP16 accessory proteinEntrez Gene (Human): 3054Entrez Gene (Mouse) : 15161SwissProt (Human): P51610SwissProt (Mouse) : Q61191Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HCFC1 expression in HeLa (A), mouse brain (B) whole cell lysates.
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Immunohistochemical analysis of HCFC1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1)