Description: Rabbit polyclonal antibody to Histone H1.0 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: H1F0 Alternative Names: H1FV; Histone H1.0; Histone H1'; Histone H1(0)Entrez Gene (Human): 3005Entrez Gene (Mouse) : 14958Entrez Gene (Rat) : 24437SwissProt (Human): P07305SwissProt (Mouse) : P10922SwissProt (Rat) : P43278Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H1.0 expression in mouse liver (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates.
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  Immunohistochemical analysis of Histone H1.0 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.