Description: Rabbit polyclonal antibody to Filamin A (Phospho-S2152) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2152 of human Filamin A protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FLNA Alternative Names: FLN; FLN1; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filaminEntrez Gene (Human): 2316SwissProt (Human): P21333Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Filamin A (Phospho-S2152) expression in HeLa calyculin A-treated (A), A431 EGF-treated (B) whole cell lysates.
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Immunohistochemical analysis of Filamin A (Phospho-S2152) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Filamin A is a phosphorylation target of membrane but not cytosolic adenylyl cyclase activity