Description: Rabbit polyclonal antibody to Cortactin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cortactin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CTTN Alternative Names: EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1Entrez Gene (Human): 2017Entrez Gene (Mouse) : 13043SwissProt (Human): Q14247SwissProt (Mouse) : Q60598Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cortactin expression in mouse kidney (A), rat lung (B) whole cell lysates.
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  Immunohistochemical analysis of Cortactin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.