Description: Rabbit polyclonal antibody to CD143 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD143. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ACE Alternative Names: DCP; DCP1; Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD143Entrez Gene (Human): 1636Entrez Gene (Mouse) : 11421Entrez Gene (Rat) : 24310SwissProt (Human): P12821SwissProt (Mouse) : P09470SwissProt (Rat) : P47820Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD143 expression in mouse kidney (A), mouse testis (B) whole cell lysates.
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  Immunohistochemical analysis of CD143 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.