Description: Rabbit polyclonal antibody to CstF-50 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CstF-50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CSTF1 Alternative Names: Cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50Entrez Gene (Human): 1477Entrez Gene (Mouse) : 67337Entrez Gene (Rat) : 311670SwissProt (Human): Q05048SwissProt (Mouse) : Q99LC2SwissProt (Rat) : Q5BJQ6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CstF-50 expression in H446 (A), mouse kidney (B) whole cell lysates.
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  Immunohistochemical analysis of CstF-50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.