Description: Rabbit polyclonal antibody to CDK8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDK8 Alternative Names: Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35Entrez Gene (Human): 1024Entrez Gene (Mouse) : 264064SwissProt (Human): P49336SwissProt (Mouse) : Q8R3L8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CDK8 expression in K562 (A), HEK293T (B), CT26 (C), HCT116 (D), EC9706 (E), PC3 (F) whole cell lysates.
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  Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.