Description: Rabbit polyclonal antibody to CDK2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDK2 Alternative Names: CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinaseEntrez Gene (Human): 1017Entrez Gene (Mouse) : 12566SwissProt (Human): P24941SwissProt (Mouse) : P97377SwissProt (Rat) : Q63699Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK2 expression in K562 (A), HeLa (B), NIH3T3 (C), rat heart (D) whole cell lysates.
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Immunohistochemical analysis of CDK2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Cyclin-C-dependent cell-cycle entry is required for activation of non-homologous end joining DNA repair in postmitotic neurons