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Anti-CDK2 Antibody CPA3170
  • Western blot analysis of CDK2 expression in K562 (A), HeLa (B), NIH3T3 (C), rat heart (D) whole cell lysates.
  • Immunohistochemical analysis of CDK2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-CDK2 Antibody
Cat No: CPA3170
Source: Rabbit
Reactivity: H, M, R, B, C, P, Rb, S, Z
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to CDK2
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK2. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: CDK2
Alternative Names: CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase
Entrez Gene (Human): 1017
Entrez Gene (Mouse) : 12566
SwissProt (Human): P24941
SwissProt (Mouse) : P97377
SwissProt (Rat) : Q63699
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CDK2 expression in K562 (A), HeLa (B), NIH3T3 (C), rat heart (D) whole cell lysates.
  • Immunohistochemical analysis of CDK2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Cyclin-C-dependent cell-cycle entry is required for activation of non-homologous end joining DNA repair in postmitotic neurons
Journal Cell Death Differ
IF 12.4
Application WB
Reactivity Rat
PMID 20111042
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