Description: Rabbit polyclonal antibody to Cyclin E1 (Phospho-T395) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T395 of human Cyclin E1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CCNE1 Alternative Names: CCNE; G1/S-specific cyclin-E1Entrez Gene (Human): 898Entrez Gene (Mouse) : 12447SwissProt (Human): P24864SwissProt (Mouse) : Q61457SwissProt (Rat) : P39949Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cyclin E1 (Phospho-T395) expression in mouse kidney (A), rat kidney (B) whole cell lysates.
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Immunohistochemical analysis of Cyclin E1 (Phospho-T395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.