Description: Rabbit polyclonal antibody to BTK (Phospho-Y223) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y223 of human BTK protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: BTK Alternative Names: AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinaseEntrez Gene (Human): 695Entrez Gene (Mouse) : 12229SwissProt (Human): Q06187SwissProt (Mouse) : P35991Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BTK (Phospho-Y223) expression in mouse spleen (A), mouse liver (B) whole cell lysates.
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  Immunofluorescent analysis of BTK (Phospho-Y223) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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  Direct ELISA antibody dose-response curve using Anti-BTK (Phospho-Y223) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.