Description: Rabbit polyclonal antibody to MASH1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MASH1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ASCL1 Alternative Names: ASH1; BHLHA46; HASH1; Achaete-scute homolog 1; ASH-1; hASH1; Class A basic helix-loop-helix protein 46; bHLHa46Entrez Gene (Human): 429Entrez Gene (Mouse) : 17172Entrez Gene (Rat) : 64186SwissProt (Human): P50553SwissProt (Mouse) : Q02067SwissProt (Rat) : P19359Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MASH1 expression in HEK293T (A), mouse lung (B), NIH3T3 (C), H9C2 (D) whole cell lysates.
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Immunohistochemical analysis of MASH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.