Description: Rabbit polyclonal antibody to Alpha-2C Adrenergic Receptor Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-2C Adrenergic Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: ADRA2C Alternative Names: ADRA2L2; ADRA2RL2; Alpha-2C adrenergic receptor; Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenoreceptor; Alpha-2C adrenoceptor; Alpha-2CAREntrez Gene (Human): 152Entrez Gene (Mouse) : 11553Entrez Gene (Rat) : 24175SwissProt (Human): P18825SwissProt (Mouse) : Q01337SwissProt (Rat) : P22086Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Alpha-2C Adrenergic Receptor expression in HEK293T (A), Hela (B), HGC27 (C), mouse kidney (D), mouse liver (E), rat kidney (F), rat liver (G) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 50 kD)
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  Immunohistochemical analysis of Alpha-2C Adrenergic Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Alpha-2C Adrenergic Receptor staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.