Description: Rabbit polyclonal antibody to ADAR1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ADAR1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ADAR Alternative Names: ADAR1; DSRAD; G1P1; IFI4; Double-stranded RNA-specific adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBPEntrez Gene (Human): 103Entrez Gene (Mouse) : 56417Entrez Gene (Rat) : 81635SwissProt (Human): P55265SwissProt (Mouse) : Q99MU3SwissProt (Rat) : P55266Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ADAR1 expression in Jurkat (A), Ramos (B), HeLa (C) whole cell lysates.
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Immunohistochemical analysis of ADAR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.