Description: Rabbit polyclonal antibody to SGK223 (Phospho-Y413) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y413 of human SGK223 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SGK223 Alternative Names: Tyrosine-protein kinase SgK223; Sugen kinase 223Entrez Gene (Human): 157285SwissProt (Human): Q86YV5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SGK223 (Phospho-Y413) expression in U251MG (A), HeLa (B) whole cell lysates.
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Immunohistochemical analysis of SGK223 (Phospho-Y413) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.