Description: Rabbit polyclonal antibody to IFN alpha 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IFN alpha 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: IFNA1; IFNA13 Alternative Names: Interferon alpha-1/13; IFN-alpha-1/13; Interferon alpha-D; LeIF DEntrez Gene (Human): 3439; 3447Entrez Gene (Mouse) : 15962Entrez Gene (Rat) : 298210SwissProt (Human): P01562SwissProt (Mouse) : P01572SwissProt (Rat) : P05011Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IFN alpha 1 expression in MEF (A), mouse spleen (B), rat spleen (C) whole cell lysates.
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  Immunohistochemical analysis of IFN alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.