Description: Rabbit polyclonal antibody to INPP5E Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human INPP5E. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: INPP5E Alternative Names: 72 kDa inositol polyphosphate 5-phosphatase; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; Phosphatidylinositol polyphosphate 5-phosphatase type IVEntrez Gene (Human): 56623Entrez Gene (Mouse) : 64436SwissProt (Human): Q9NRR6SwissProt (Mouse) : Q9JII1SwissProt (Rat) : Q9WVR1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of INPP5E expression in HEK293T (A), SP2/0 (B), H9C2 (C) whole cell lysates.
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  Immunofluorescent analysis of INPP5E staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).