Description: Rabbit polyclonal antibody to UBR2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UBR2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: UBR2 Alternative Names: C6orf133; KIAA0349; E3 ubiquitin-protein ligase UBR2; N-recognin-2; Ubiquitin-protein ligase E3-alpha-2; Ubiquitin-protein ligase E3-alpha-IIEntrez Gene (Human): 23304Entrez Gene (Mouse) : 224826SwissProt (Human): Q8IWV8SwissProt (Mouse) : Q6WKZ8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBR2 expression in HepG2 (A), mouse liver (B), rat liver (C) whole cell lysates.
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  Immunohistochemical analysis of UBR2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.