Description: Rabbit polyclonal antibody to SEC61B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SEC61B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SEC61B Alternative Names: Protein transport protein Sec61 subunit betaEntrez Gene (Human): 10952Entrez Gene (Mouse) : 66212SwissProt (Human): P60468SwissProt (Mouse) : Q9CQS8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SEC61B expression in rat kidney (A), rat liver (B) whole cell lysates.
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Immunohistochemical analysis of SEC61B staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.