Description: Rabbit polyclonal antibody to TRPV1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TRPV1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: TRPV1 Alternative Names: VR1; Transient receptor potential cation channel subfamily V member 1; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1Entrez Gene (Human): 7442Entrez Gene (Mouse) : 193034Entrez Gene (Rat) : 83810SwissProt (Human): Q8NER1SwissProt (Mouse) : Q704Y3SwissProt (Rat) : O35433Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRPV1 expression in U87MG (A), A375 (B) whole cell lysates.
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  Immunofluorescent analysis of TRPV1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).