Description: Rabbit polyclonal antibody to SPARC Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPARC. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SPARC Alternative Names: ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteineEntrez Gene (Human): 6678Entrez Gene (Mouse) : 20692Entrez Gene (Rat) : 24791SwissProt (Human): P09486SwissProt (Mouse) : P07214SwissProt (Rat) : P16975Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SPARC expression in A549 (A), mouse kidney (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of SPARC staining in mouse muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.