Description: Rabbit polyclonal antibody to PKLR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKLR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PKLR Alternative Names: PK1; PKL; Pyruvate kinase PKLR; Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinaseEntrez Gene (Human): 5313Entrez Gene (Rat) : 24651SwissProt (Human): P30613SwissProt (Mouse) : P53657SwissProt (Rat) : P12928Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PKLR expression in DLD (A), mouse muscle (B), rat muscle (C) whole cell lysates.
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Immunofluorescent analysis of PKLR staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).