Description: Rabbit polyclonal antibody to CD89 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD89. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FCAR Alternative Names: CD89; Immunoglobulin alpha Fc receptor; IgA Fc receptor; CD89Entrez Gene (Human): 2204SwissProt (Human): P24071Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD89 expression in THP1 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
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Immunohistochemical analysis of CD89 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.