Description: Rabbit polyclonal antibody to GM-CSF Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GM-CSF. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CSF2 Alternative Names: GMCSF; Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; SargramostimEntrez Gene (Human): 1437Entrez Gene (Mouse) : 12981Entrez Gene (Rat) : 116630SwissProt (Human): P04141SwissProt (Mouse) : P01587SwissProt (Rat) : P48750Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GM-CSF expression in mouse muscle (A), rat muscle (B) whole cell lysates.
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  Immunohistochemical analysis of GM-CSF staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.