Description: Rabbit polyclonal antibody to GPR125 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR125. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GPR125 Alternative Names: Probable G-protein coupled receptor 125Entrez Gene (Human): 166647Entrez Gene (Mouse) : 70693SwissProt (Human): Q8IWK6SwissProt (Mouse) : Q7TT36Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GPR125 expression in Hela (A), A549 (B), mouse muscle (C), rat muscle (D) whole cell lysates.
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Immunohistochemical analysis of GPR125 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.