Description: Rabbit polyclonal antibody to TRPV3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRPV3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TRPV3 Alternative Names: Transient receptor potential cation channel subfamily V member 3; TrpV3; Vanilloid receptor-like 3; VRL-3Entrez Gene (Human): 162514SwissProt (Human): Q8NET8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TRPV3 expression in U87MG (A), MCF7 (B), C6 (C), CT26 (D) whole cell lysates.
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Immunohistochemical analysis of TRPV3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.