Description: Rabbit polyclonal antibody to SYNE3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SYNE3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: SYNE3 Alternative Names: C14orf49; Nesprin-3; Nuclear envelope spectrin repeat protein 3Entrez Gene (Human): 161176Entrez Gene (Mouse) : 212073SwissProt (Human): Q6ZMZ3SwissProt (Mouse) : Q4FZC9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SYNE3 expression in AML12 (A), H9C2 (B), A549 (C), HepG2 (D), HEK293T (E) whole cell lysates.
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  Immunofluorescent analysis of SYNE3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.