Description: Rabbit polyclonal antibody to NCOA7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NCOA7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NCOA7 Alternative Names: ERAP140; ESNA1; Nuclear receptor coactivator 7; 140 kDa estrogen receptor-associated protein; Estrogen nuclear receptor coactivator 1Entrez Gene (Human): 135112Entrez Gene (Mouse) : 211329SwissProt (Human): Q8NI08SwissProt (Mouse) : Q6DFV7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NCOA7 expression in K562 (A), mouse liver (B), mouse kidney (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of NCOA7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.