Description: Rabbit polyclonal antibody to Caspase 12 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 12. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CASP12 Alternative Names: Inactive caspase-12; CASP-12Entrez Gene (Human): 100506742Entrez Gene (Mouse) : 12364Entrez Gene (Rat) : 156117SwissProt (Human): Q6UXS9SwissProt (Mouse) : O08736SwissProt (Rat) : Q920D5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Caspase 12 expression in HEK293T (A), A549 (B), U2OS (C), mouse brain (D), mouse spleen (E), rat brain (F) whole cell lysates.
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Immunohistochemical analysis of Caspase 12 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.