Anti-LRP11 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates.
Immunoprecipitation of LRP11 from 0.5mg Jurkat whole cell extract lysate, using 5ug of Anti-LRP11 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-LRP11 Antibody.

Product Name	Anti-LRP11 Antibody
Cat No:	CPA2823
Source:	Rabbit
Reactivity:	H
Applications:	WB, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Rabbit polyclonal antibody to LRP11

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LRP11. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/100)

Gene Symbol: LRP11

Alternative Names: Low-density lipoprotein receptor-related protein 11; LRP-11

Entrez Gene (Human): 84918

SwissProt (Human): Q86VZ4

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates.
Immunoprecipitation of LRP11 from 0.5mg Jurkat whole cell extract lysate, using 5ug of Anti-LRP11 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-LRP11 Antibody.

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