Anti-Histone Deacetylase 10 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Histone Deacetylase 10 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone Deacetylase 10. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: HDAC10 Alternative Names: Histone deacetylase 10; HD10Entrez Gene (Human): 83933Entrez Gene (Mouse) : 170787Entrez Gene (Rat) : 362981SwissProt (Human): Q969S8SwissProt (Mouse) : Q6P3E7SwissProt (Rat) : Q569C4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Histone Deacetylase 10 expression in rat live (A), rat kidney (B) whole cell lysates. -
Immunohistochemical analysis of Histone Deacetylase 10 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of Histone Deacetylase 10 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases
Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus