Description: Rabbit polyclonal antibody to CD318 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD318. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CDCP1 Alternative Names: TRASK; CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD318Entrez Gene (Human): 64866Entrez Gene (Mouse) : 109332SwissProt (Human): Q9H5V8SwissProt (Mouse) : Q5U462Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD318 expression in Hela (A), mouse kidney (B), rat lung (C) whole cell lysates.
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Immunohistochemical analysis of CD318 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.