Description: Rabbit polyclonal antibody to Artemis (Phospho-S516) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S516 of human Artemis protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: DCLRE1C Alternative Names: ARTEMIS; ASCID; SCIDA; SNM1C; Protein artemis; DNA cross-link repair 1C protein; Protein A-SCID; SNM1 homolog C; hSNM1C; SNM1-like proteinEntrez Gene (Human): 64421Entrez Gene (Mouse) : 227525Entrez Gene (Rat) : 259171SwissProt (Human): Q96SD1SwissProt (Mouse) : Q8K4J0SwissProt (Rat) : Q5XIX3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Artemis (Phospho-S516) expression in Hela (A), H446 (B), H1688 (C), mouse lung (D) whole cell lysates.
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Immunohistochemical analysis of Artemis (Phospho-S516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.