Description: Rabbit polyclonal antibody to NEIL3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NEIL3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NEIL3 Alternative Names: Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3Entrez Gene (Human): 55247Entrez Gene (Mouse) : 234258SwissProt (Human): Q8TAT5SwissProt (Mouse) : Q8K203Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NEIL3 expression in MCF7 (A), H1792 (B), rat thymus (C), mouse testis (D) whole cell lysates.
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Immunohistochemical analysis of NEIL3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.