Description: Rabbit polyclonal antibody to SAP14 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SAP14. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: SF3B14 Alternative Names: SAP14; SF3B14; SF3B14A; Splicing factor 3B subunit 6; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6, 14kDaEntrez Gene (Human): 51639Entrez Gene (Mouse) : 66055SwissProt (Human): Q9Y3B4SwissProt (Mouse) : P59708Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SAP14 expression in HEK293T (A), HepG2 (B), AML12 (C), PC12 (D) whole cell lysates.
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Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.