Description: Rabbit polyclonal antibody to ATP6V1H Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ATP6V1H Alternative Names: V-type proton ATPase subunit H; V-ATPase subunit H; Nef-binding protein 1; NBP1; Protein VMA13 homolog; V-ATPase 50/57 kDa subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFDEntrez Gene (Human): 51606Entrez Gene (Mouse) : 108664SwissProt (Human): Q9UI12SwissProt (Mouse) : Q8BVE3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATP6V1H expression in H9C2 (A), MEF (B), A549 (C), HepG2 (D) whole cell lysates.
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  Immunohistochemical analysis of ATP6V1H staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.