Description: Rabbit polyclonal antibody to A4GNT Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A4GNT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: A4GNT Alternative Names: Alpha-1,4-N-acetylglucosaminyltransferase; Alpha4GnTEntrez Gene (Human): 51146SwissProt (Human): Q9UNA3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of A4GNT expression in HEK293T (A), mouse testis (B), rat kidney (C) whole cell lysates.
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Immunohistochemical analysis of A4GNT staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.